Journal: bioRxiv

Article Title: FoQDE2-dependent milRNA promotes Fusarium oxysporum f. sp. cubense virulence by targeting a glycosyl hydrolase coding gene at transcriptional level

doi: 10.1101/2021.12.02.470887

Figure Lengend Snippet: Double-strand siRNAs (siRNA-1 and siRNA-2) and single-strand inhibitor against the precursor sequence of milR-87, as well as a random small RNA (24 nt) serving as a negative control (NC) were synthesized and transfected into Foc with aid of lipofectamine 2000 (Invitrogen). After 2 days transfection, conidia suspensions of the transfected strains and positive control of the ΔmilR-87 mutant, as well as non-transfected strain of Foc (CK) were inoculated on the banana leaves. After 3 days, the lesions on banana leaves were photographed. ( A ) Symptoms of pathogenicity on the host banana leaves. ( B ) Lesions sizes were measured for accessing virulence of the different strains. Error bars indicate S. D. (n=3). *, p<0.05; **, p<0.01. ( C ) Relative expression of milR-87 in the transfected strains, the non-transfected strain of Foc (CK), and the ΔmilR-87 mutant. The total RNAs of the tested strains cultured at 28°C for 7 days were extracted for milRNA detection by qRT-PCR. Relative expression levels of milR-87 in the transfected strains were normalized to that of non-transfected strain of Foc (CK), which was set as 1. snRNA U4 was used as internal control. Error bars indicate S. D. (n=3). **, p<0.01.

Article Snippet: Double-strand siRNAs (siRNA-1 and siRNA-2) and single-strand inhibitor against the precursor sequence of milR-87, as well as a random sRNA sequence (24 nt) serving as a negative control (NC) were designed and synthesized by General Biosystems company.

Techniques: Sequencing, Negative Control, Synthesized, Transfection, Positive Control, Mutagenesis, Expressing, Cell Culture, Quantitative RT-PCR, Control

Journal: Pilot and Feasibility Studies

Article Title: Impact of a multimedia website with patient experiences of multiple sclerosis (PExMS) on immunotherapy decision-making: study protocol for a pilot randomised controlled trial in a mixed-methods design

doi: 10.1186/s40814-020-00749-0

Figure Lengend Snippet: Study assessment

Article Snippet: The concealed allocation sequence will be computer-generated by the randomisation sequence of QuestBack Unipark.

Techniques: